Device

Part:BBa_K1627006:Design

Designed by: Alejandro Gutierrez   Group: iGEM15_Austin_UTexas   (2015-09-10)


Device to demethylate caffeine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1733
    Illegal NheI site found at 1928
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2684
    Illegal BglII site found at 3180
    Illegal BglII site found at 3651
    Illegal XhoI site found at 4792
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5490
    Illegal AgeI site found at 3319
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 983


Design Notes

These genes were amplified from the pDCAF3 plasmid (BBa_K734000). A PstI restriction site was removed from the ndmA gene and an EcoRI restriction site was removed from ndmB during the cloning process. The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly.


Source

The ndmA, ndmB, ndmC, and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.

References